Flow Cytometry Network

AbstractThe 3D spatial organization of genes and other genetic elements within the nucleus is important for regulating gene expression. Understanding how this spatial organization is established and maintained throughout the life of a cell is key to ...

AbstractNeutrophils expel extracellular traps (NETs) to entrap and exterminate the invaded micro-organisms. Acute/chronic inflammatory disorders are often observed with aberrantly enhanced NETs formation and high nitric oxide (NO) availability. Recent ...

AbstractMicrobial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and ultimately for its quantification. Some of the major limitations found within these ...

AbstractIn cerebrospinal fluid (CSF) analysis, hematology analyzers (HAs) Sysmex® XT-4000i and XE-5000, equipped with flow cytometry (FCM), were used to count cells and differentiate leukocytes into mononuclear and polymorphonuclear cells (MNCs, PMCs) ...

AbstractAcute promyelocytic leukemia (APL) is a hematological emergency in which a rapid diagnosis is essential for early administration of appropriate therapy, including all-trans retinoic acid before the onset of fatal coagulopathy. Currently, the ...

AbstractAmyloid beta (Aβ) oligomers and phosphorylated tau (p-tau) aggregates are increasingly identified as potential toxic intermediates in Alzheimer's disease (AD). In cortical AD synapses, p-tau co-localizes with Aβ, but the Aβ and p-tau peptide ...

AbstractOn the cover: The image for the cover of this issue displays results from application of a nucleus segmentation algorithm (see Figure 2 in this issue's accompanying article by Ram et al.)Cover design by Bärbel Beran [www.beran-design.de].

AbstractA proposal for a scoring system in the diagnosis of chronic lymphoproliferative diseases other than CLL has been recently published in Cytometry Part B. The authors apply this score for deciding whether or not FISH evaluation for the detection ...

AbstractIntroduction:Expression of ZAP-70 by chronic lymphocytic leukemia (CLL) is associated with more aggressive disease and can help differentiate CLL using mutated immunoglobulin heavy chain variable genes (VH) from cases expressing unmutated VH ...

AbstractBackground:CD80 is a member of the B7 family of immune coregulatory proteins that mediate both immune activation and suppression. CD80 in particular has recently been shown to play an important role in supporting immune suppression through ...

AbstractBackground:Differential cell count in bronchoalveolar lavage (BAL) has an important role in the diagnosis of pulmonary diseases. Optical microscopy method is usually chosen to identify BAL leukocyte populations despite its technical limitations. ...

AbstractAims and objectives:To build an artificial neural network (ANN) model for the detection of carcinoma in effusion cytology.Materials and methods:We selected a total of 114 effusion cytology cases consisting of 57 each benign and malignant case. ...

AbstractBasophils are key effector cells in allergic inflammatory reactions. However, the mechanisms of FcεRI-induced degranulation are complex and remain to be disentangled. Signal transducer and activator of transcription (STAT) molecules modulate ...

AbstractBackground:Flow cytometric immunophenotyping has an established role in the diagnosis and monitoring of B-lymphoblastic leukemia (B-LL). However, the search continues for an optimal reagent set that can identify leukemic blasts with specificity, ...

Abstract:  This protocol describes methods for monitoring intracellular phosphorylation‐dependent signaling events on a single‐cell basis. This approach measures cell signaling by treating ...

Abstract:  Long-term benefits of cardiovascular therapeutic interventions are often reduced due to reocclusion of the treated vessels. Therefore, a therapeutic strategy to reduce neointima formation ...

Abstract:  This unit describes the use of a novel violet-excited membrane-binding probe F2N12S [4¢-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone] for the flow ...

Abstract:  In flow cytometry, the fluorescence decay time of an excitable species has been largely underutilized and is not likely found as a standard parameter on any imaging cytometer, sorting, or ...

Abstract:  Traditional approaches for translating observations of molecular events into the context of a living organism have suffered from the requirements for either sacrificing animals at multiple ...

Abstract:  Antibodies are instrumental tools in stem cell identification, purification, and analysis. Most commonly, cell samples are either dissociated to obtain a single-cell suspension suitable for ...

Abstract:  Embryonic stem (ES) cell lines are derived from the inner cell mass of the pre-implantation blastocyst and are characterized by the ability to undergo indefinite self-renewal while retaining ...

Abstract:  The objective is the most crucial image-forming component of a microscope. A knowledge of the many types of objectives available and their characteristics is critical to the selection of ...

Abstract:  Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular ...

Abstract:  Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a ...

L-selectin and SDF-1 enhance the migration of mouse and human cardiac mesoangioblasts Cell Death and Differentiation 19, 345 (February 2012). doi:10.1038/cdd.2011.110 Authors: A Bernal, N San Martín, M Fernández, D ...

Regulation of skin aging and heart development by TAp63 Cell Death and Differentiation 19, 186 (February 2012). doi:10.1038/cdd.2011.181 Authors: M Paris, M Rouleau, M Pucéat & D Aberdam

Selective regulation of IP3-receptor-mediated Ca2+ signaling and apoptosis by the BH4 domain of Bcl-2 versus Bcl-Xl Cell Death and Differentiation 19, 295 (February 2012). doi:10.1038/cdd.2011.97 Authors: G Monaco, E Decrock, H ...

Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems Cell Death and Differentiation 19, 194 (February 2012). doi:10.1038/cdd.2011.141 Authors: F Cesca, A Yabe, B ...

TNF-α response of vascular endothelial and vascular smooth muscle cells involve differential utilization of ASK1 kinase and p73 Cell Death and Differentiation 19, 274 (February 2012). doi:10.1038/cdd.2011.93 Authors: S Rastogi, W ...

Jagged2 controls the generation of motor neuron and oligodendrocyte progenitors in the ventral spinal cord Cell Death and Differentiation 19, 209 (February 2012). doi:10.1038/cdd.2011.84 Authors: M A Rabadán, J Cayuso, G Le ...

Tankyrase-1 function at telomeres and during mitosis is regulated by Polo-like kinase-1-mediated phosphorylation Cell Death and Differentiation 19, 321 (February 2012). doi:10.1038/cdd.2011.101 Authors: G-H Ha, H-S Kim, H Go, H Lee, H ...

Transcription factor TEAD4 regulates expression of Myogenin and the unfolded protein response genes during C2C12 cell differentiation Cell Death and Differentiation 19, 220 (February 2012). doi:10.1038/cdd.2011.87 Authors: A ...

Resuscitating supplementary information: is the solution a two-for-one offer? Cell Death and Differentiation 19, 185 (February 2012). doi:10.1038/cdd.2011.177 Authors: R A Knight & G Melino

The miR 302-367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG network Cell Death and Differentiation 19, 232 ...